|
Research Assistant/Post Doctoral Scientist Positions
Phylogica is an Australian-based biotechnology company that is applying its proprietary Phylomer® drug discovery platform to develop novel peptide-based therapies for human diseases. Phylogica has recently announced a collaboration with Roche and is currently expanding its relationships with other pharmaceutical partners. Phylogica is listed on the ASX (PYC) and is based at the Telethon Institute of Child Health Research in Perth, Western Australia.
We are seeking highly motivated, ambitious and skilled individuals to fill Research Assistant and Post Doctoral Scientist positions in our Protein expression, Bioassay and Phage display groups.. Details regarding the selection criteria for each position can be found at www.phylogica.com. The successful applicants would be part of a young and dynamic research team working at the cutting edge of drug discovery. The company will negotiate competitive salary packages with successful applicants.
Applications close 12th July, 2010.
For further information please contact Farzana Khan (farzanak@phylogica.com) on (08) 9489 7894. Applications should indicate the position being applied for and include a statement addressing the respective selection criteria, a curriculum vitae and the contact details of two referees and be forwarded to: Julia Emmerson, Human Resources Manager, Telethon Institute for Child Health Research, PO Box 855, West Perth, WA, 6872. Selection criteria can also be obtained by contacting reception (Ph: 9489 7777; email: reception@ichr.uwa.edu.au.)
Selection Criteria Position 1: Research Assistants (Molecular Biology with Antimicrobial Focus)
An experienced Research Assistant with experience in Molecular Biology
Activities will include:
- Cloning and expression of proteins/peptides or single-chain antibodies
- Analysis of proteins using ELISA, SDS-PAGE electrophoresis and immuno-blotting, small-scale protein purification
- DNA sequence analysis and bioinformatic analysis of derived peptide sequences/structures
- Construction of peptide libraries in bacteriophage and yeast
- Screening of peptide libraries using genetic screening approaches such as phage display
- Characterisation of peptide/protein interactions using ELISA
- Bacterial inhibition (MIC) tests, antibiotic resistance tests
- Contributing to team activities for maintenance of laboratory and database management
Essential
- A B.Sc (Hons) in appropriate disciplines such as Molecular Biology or biochemistry
- At least 2-4 years post-degree experience involving successful application involving a range of appropriate Molecular Biology and protein expression techniques, including DNA amplification and cloning, DNA and RNA preparation, PCR and restriction analysis
- Excellent written and oral communication skills
- Excellent organisational skills and ability to maintain accurate research records
- Enthusiasm to work on commercially-orientated project
- Ability to work independently
Desirable
- Experience with high-throughput screening methods such as phage display
- Previous experience with protein expression
- Microbiology skills or experience with bacterial inhibition tests
Selection Criteria Position 2: Postdoctoral Scientist (Molecular Biology with Antimicrobial Focus)
An experienced Post Doctoral Scientist is required with extensive experience in Molecular Biology and/or microbiology
Activities will include:
- Cloning and expression of proteins/peptides or single-chain antibodies; sourcing of suitable vectors and expression strains
- Analysis of proteins using ELISA, SDS-PAGE electrophoresis and immuno-blotting, small-scale protein purification
- DNA sequence analysis and bioinformatic analysis of derived peptide sequences/structures
- Construction of peptide libraries in bacteriophage and yeast
- Screening of peptide libraries using genetic screening approaches such as phage display
- Characterisation of peptide/protein interactions using ELISA
- Bacterial inhibition (MIC) tests, antibiotic resistance tests
- Contributing to team activities for maintenance of laboratory and database management
Essential
- PhD degree in an appropriate disciplines such as biochemistry, biotechnology, microbiology or molecular biology
- For PhD applicants, demonstrated experience involving successful application of a range of appropriate molecular biology and proteomic techniques
- Excellent written and oral communication skills
- Excellent organisational and time-planning skills; ability to maintain accurate research records
- Enthusiasm to work on commercially-orientated project
- Ability to work independently
Desirable
- Experience with high-throughput screening methods such as phage or yeast display
- Previous experience with protein expression, particularly secretion from yeast
- Microbiology skills and experience with bacterial inhibition tests
- Protein analysis techniques such as ELISA, SDS-PAGE electrophoresis and immunoblotting
Selection Criteria Position 3 – Postdoctoral Scientist (Protein expression)
A Postdoctoral Scientist with experience in molecular biology and recombinant protein expression
Activities will include:
- Cloning and Expression of proteins/peptides
- Protein/peptide purification using various chromatography techniques
- Analysis of proteins using ELISA, SDS-PAGE electrophoresis
- Analysis of peptides/proteins using HPLC
- Analysis of protein/peptide interactions and structures using various biophysical techniques including Biacore, Isothermal Titration Calorimetry and Circular Dichroism.
- Bioinformatic analysis of peptide sequences/structures.
- Contributing to team activities for maintenance of laboratory and database management
- Data presentation in a group setting
Essential
- A PhD in an appropriate discipline such as biochemistry and protein chemistry
- At least 2-4 years post-doctoral experience in the appropriate Molecular Biology and Protein Expression Techniques
- Extensive experience in protein chromatography (AKTA FPLC platform)
- Excellent written and oral communication skills
- Excellent organisational skills and ability to maintain accurate research records
- Enthusiasm to work on commercially-orientated project
- Ability to work independently
Desirable
- Molecular biology techniques such as DNA and RNA preparation, PCR, restriction analysis and DNA cloning
- Previous experience with protein/protein interaction techniques and/or protein structure determination (protein crystallography and NMR)
- Experience in the supervision of Research Assistants
Selection Criteria Position 4 – Research Assistant (Protein expression)
A Research Assistant with experience in molecular biology and recombinant protein expression
Activities will include:
- Cloning and Expression of proteins/peptides
- Protein/peptide purification using various chromatography techniques
- Analysis of proteins using ELISA, SDS-PAGE electrophoresis
- Assist with analysis of peptides/proteins using FPLC/HPLC
- Analysis of protein/peptide interactions and structures using various biophysical techniques including Biacore, Isothermal Titration Calorimetry and Circular Dichroism.
- Bioinformatic analysis of peptide sequences/structures.
- Contributing to team activities for maintenance of laboratory and database management
- Data presentation in a group setting
Essential
- A B.Sc (Hons) in an appropriate discipline such as biochemistry and protein chemistry
- At least 2-4 years post-degree experience in the appropriate Molecular Biology and Protein Expression Techniques
- Excellent written and oral communication skills
- Excellent organisational skills and ability to maintain accurate research records
- Enthusiasm to work on commercially-orientated project
- Ability to work independently
Desirable
- Molecular biology techniques such as DNA and RNA preparation, PCR, restriction analysis and DNA cloning
- Previous experience with protein/protein interaction techniques and/or protein structure determination (protein crystallography and NMR)
- Experience in protein chromatography
Selection Criteria Position 5 – Research Assistant (Molecular Biology/Phage display)
Activities will include:
- Screening of peptide libraries using genetic screening approaches such as phage display
- Construction of large-scale peptide libraries in various formats
- PCR amplification and sequence analysis of peptide DNA inserts
- Bioinformatic analysis of peptide sequences/structures
- Analysis of phage displayed and synthetic peptide/protein interactions using ELISA-based approaches
- Contributing to team activities for maintenance of laboratory and database management
Essential
- A B.Sc (Hons) in appropriate disciplines such as molecular biology or biochemistry
- At least 2-4 years post-degree experience
- Extensive experience and knowledge in a wide range of appropriate Molecular Biology techniques, including general cloning strategies, DNA and RNA preparation, PCR and restriction analysis, ELISA
- Excellent written and oral communication skills
- Excellent organisational skills and ability to maintain accurate research records
- Enthusiasm to work on commercially-orientated project
- Ability to work independently
Desirable
- Experience with HTP screening methods such as phage display
- Previous experience with recombinant protein expression techniques
- Experience with in vitro culture techniques
Selection Criteria Position 6 – Research Assistant (Bioassay Development/Screening)
Qualifications and Experience:
Will have relevant undergraduate or postgraduate qualifications and several years of laboratory experience including a range of multidisciplinary skills, encompassing in vitro cell culture, basic molecular biology techniques and an appreciation for good laboratory practise.
Duties:
- A variety of in vitro immunological assays, such as tissue culture and measurement of cell products using proliferative, ELISA and real-time PCR assays.
- Perform a variety of in vivo immunological assays.
- Analysis of data using statistical and database computer packages
- Participation in the planning of experiments
- Undertake laboratory work consistent with the requirement of Good Laboratory Practice
- Assist with the preparation of publications and reports
- Maintain current knowledge of scientific literature relating to the project
- Liaising with other team members across Commercial Ready group
Essential
- A BSc (Hons) in appropriate disciplines such as Molecular Biology, Biochemistry, or Immunology
- Minimum 2-4 years post-degree experience
- Experience involving a range of immunological techniques, including in vivo assay implementation and sample collection
- Experience in the analysis of samples by ELISA, FACS and proliferative assay techniques
- Experience in tissue culture techniques
- Experience involving a range of molecular biology techniques, such as DNA amplification and cloning, DNA and RNA purification, PCR and restriction analysis
- Excellent written and oral communication skills
- Excellent computer skills
- Excellent organisational skills
- Enthusiasm to work on commercially-orientated project
- Ability to work independently
Desirable
- Experience in real-time PCR
- Experience in yeast genetics and gene expression
|
