Phylomer^® library formats and drug discovery screening

Phylogica has developed a panel of Phylomer^® libraries, which are tailor-made to allow screening depending upon the nature of the target - be it extracellular or intracellular.

Phylomer^® libraries are in various phage display and yeast-two-hybrid formats as well as in mammalian expression vectors for phenotypic screening. Suitable for high throughput, these platforms facilitate rapid sampling of the Phylomer^® libraries for the structures and shapes that most efficiently bind a protein or disrupt specific protein interactions that may be important mediators of disease.

Phylogica has successfully screened its Phylomer^® libraries to isolate potent Phylomer^® peptide leads against:

• Challenging intracellular targets, including signalling adaptor proteins and transcription factors, and
• Extracellular biological drug targets, receptors-ligand interactions.

Phylomer^® libraries have also yielded high quality hits in cell-based screens for binders to membrane-associated targets.

Case Studies

Click on the links below to view case studies of Phylomer^® applications:

• Screening extracellular targets using phage display
• Cell-based screening using phage display
• Discovery of customised scaffolds from Phylomer^® libraries
• Screening of intracellular targets using yeast-two-hybrid assays
• Screening for novel cell-penetrating Phylomers^®